Cholera Toxin beta antibody (HRP)

Catalog No. ABIN1710863

Product Details anti-Cholera Toxin beta Antibody (HRP)

Target: Cholera Toxin beta

Reactivity: Vibrio cholerae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cholera Toxin beta antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Rat

Cross-Reactivity (Details): Cholera Toxin Subunit B

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from CTXB/beta subunit Cholera Toxin

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Cholera Toxin beta

Target: Cholera Toxin beta

Abstract: Cholera Toxin beta Products

Background:

Synonyms: Cholera enterotoxin B chain, Cholera enterotoxin beta chain, Cholera enterotoxin gamma chain, Cholera enterotoxin subunit B, Cholera toxin B protein, Choleragenoid, CTX B, CTXB, TOX B, TOXB, VC1456, CHTB_VIBCH.

Background: The B subunit of cholera toxin (CtxB) binds to a GM1-ganglioside receptor, a ubiquitous glycolipid cell surface receptor. This binding is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin A subunit into cells. The beta chain has no toxic activity by itself. The holotoxin consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The A subunit (and Cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.

Gene ID: 2613962