HHATL antibody (AA 25-125) (Biotin)

Catalog No. ABIN1701329

Product Details anti-HHATL Antibody (Biotin)

Target: HHATL (Hedgehog Acyltransferase-Like (HHATL))

Binding Specificity: AA 25-125

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HHATL antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HHATL/GUP1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for HHATL

Target: HHATL (Hedgehog Acyltransferase-Like (HHATL))

Alternative Name: HHATL/GUP1 (HHATL Products)

Synonyms: Gup1 antibody, RGD1311911 antibody, gup1 antibody, oact3 antibody, mboat3 antibody, MGC53261 antibody, mstp002 antibody, MGC89797 antibody, GUP1 antibody, 1110011D13Rik antibody, C3orf3 antibody, MBOAT3 antibody, MSTP002 antibody, OACT3 antibody, c3orf3b antibody, gup1b antibody, im:7145545 antibody, wu:fc04e05 antibody, zgc:154131 antibody, c3orf3a antibody, gup1a antibody, zgc:77750 antibody, hedgehog acyltransferase-like antibody, hedgehog acyltransferase like S homeolog antibody, hedgehog acyltransferase like antibody, hedgehog acyltransferase like, b antibody, hedgehog acyltransferase like, a antibody, Hhatl antibody, hhatl.S antibody, HHATL antibody, hhatl antibody, hhatlb antibody, hhatla antibody

Background:

Synonyms: C3orf3, Glycerol uptake/transporter homolog, GUP1, GUP1 glycerol uptake/transporter homolog, Hedgehog acyltransferase like, Hedgehog acyltransferase-like protein, Hhatl, HHATL_HUMAN, KIAA1173, MBOAT3, Membrane bound O acyltransferase domain containing 3, MSTP002, OACT3, Protein-cysteine N-palmitoyltransferase HHAT-like protein.

Background: GUP1 is a 504 amino acid multipass membrane protein of the endoplasmic reticulum that functions as a membrane bound O-acyltransferase. With specific expression in heart, GUP1 negatively regulates amino-terminal palmitoylation of Shh by HHAT, a protein that is required for Shh signaling. Deletion of the gene encoding GUP1 results in higher sensibility to specific sphinogolipid biosynthesis inhibitors and resistance to ergosterol biosynthesis inhibitors, indicating that GUP1 is an essential component in lipid metabolism. Also, GUP1 also seems to be important for cell wall assembly and stability due to evidence in Saccharomyces cerevisiae GUP1 mutants, which exhibit altered plasma membrane lipid composition and membrane potential.

Gene ID: 57467

Pathways: Hedgehog Signaling