ALKBH1 antibody (AA 1-100) (Biotin)

Catalog No. ABIN1701147

Product Details anti-ALKBH1 Antibody (Biotin)

Target: ALKBH1 (AlkB, Alkylation Repair Homolog 1 (ALKBH1))

Binding Specificity: AA 1-100

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALKBH1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ABH1/ALKB

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for ALKBH1

Target: ALKBH1 (AlkB, Alkylation Repair Homolog 1 (ALKBH1))

Alternative Name: ABH1/ALKB (ALKBH1 Products)

Synonyms: Alkbh antibody, ABH antibody, ABH1 antibody, ALKBH antibody, alkB antibody, hABH antibody, 2700073G19Rik antibody, Abh antibody, alkB homolog 1, histone H2A dioxygenase antibody, Alkbh1 antibody, ALKBH1 antibody

Background:

Synonyms: ABH, ABH1, alkB, AlkB, alkylation repair homolog 1 E. coli, ALKB1_HUMAN, ALKBH, ALKBH1, Alkylated DNA repair protein alkB homolog 1, Alkylation repair homolog 1, Alkylation repair, alkB homolog, Alpha-ketoglutarate-dependent dioxygenase ABH1, DNA lyase ABH1, hABH.

Background: ALKB protects DNA and RNA against damage from methylating compounds from the environment by directly reversing 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) cytotoxic alkylation lesions in DNA and RNA. The enzymes act by oxidative demethylation, utilizing ferrous iron and alpha-ketoglutarate as cofactors, 2-oxoglutarate as a co-substrate, and molecular oxygen as the oxidizing agent. Deficiencies in DNA and RNA repair in mammals are associated with cancer, neurological disease and developmental defects. ALKB plays a role in resistance to anti-cancer drugs which attempt to damage tumor DNA. Escherichia coli ALKB protein belongs to the superfamily of 2-oxoglutarate- and iron(II)-dependent oxygenases.

Gene ID: 8846

Pathways: DNA Damage Repair