DCLRE1C antibody (AA 301-400) (Biotin)

Catalog No. ABIN1700852

Product Details anti-DCLRE1C Antibody (Biotin)

Target: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Binding Specificity: AA 301-400

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCLRE1C antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DCLREC1C/Artemis

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for DCLRE1C

Target: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Alternative Name: DCLREC1C/Artemis (DCLRE1C Products)

Synonyms: artemis antibody, A-SCID antibody, DCLREC1C antibody, RS-SCID antibody, SCIDA antibody, SNM1C antibody, hSNM1C antibody, Snm1l antibody, nuclease antibody, 9930121L06Rik antibody, AI661365 antibody, Art antibody, DNA cross-link repair 1C antibody, DCLRE1C antibody, Dclre1c antibody

Background:

Synonyms: SCIDA, SNM1C, A-SCID, RS-SCID, DCLREC1C, Protein artemis, DNA cross-link repair 1C protein, Protein A-SCID, SNM1 homolog C, hSNM1C, SNM1-like protein, DCLRE1C, ARTEMIS, ASCID

Background: Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.

Gene ID: 64421

UniProt: Q96SD1

Pathways: DNA Damage Repair