EEF2K antibody (pSer359) (Biotin)

Catalog No. ABIN1700532

Product Details anti-EEF2K Antibody (Biotin)

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Binding Specificity: pSer359

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2K antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Sheep,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human EEF2k around the phosphorylation site of Ser359

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for EEF2K

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Alternative Name: EEF2k (EEF2K Products)

Synonyms: HSU93850 antibody, eEF-2K antibody, C86191 antibody, SMEF2K antibody, eEF-2 kinase antibody, cb365 antibody, fa04b08 antibody, wu:fa04b08 antibody, zgc:100973 antibody, eukaryotic elongation factor 2 kinase antibody, eukaryotic elongation factor-2 kinase antibody, EEF2K antibody, Eef2k antibody, eef2k antibody, LOC101930123 antibody

Background:

Synonyms: EEF2K phospho S359, p-EEF2K phospho S359, Calcium/calmodulin dependent eukaryotic elongation factor 2, Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase, Calmodulin dependent protein kinase III, cb365, eEF 2 kinase, eEF 2K, EEF2K protein, Elongation factor 2 kinase, Eukaryotic elongation factor 2 kinase, kinase eEF2K, SMEF2K, EF2K_HUMAN.

Background: Eukaryotic elongation factor 2 kinase (EEF2k) previously known as Ca2+/calmodulin dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor 2 (eEf2). Phosphorylation of eEF2 by eEF2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation. The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively via phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogensis and malignant tumors.

Gene ID: 29904

Pathways: AMPK Signaling