SUMO2/3 antibody (AA 21-95) (Biotin)

Catalog No. ABIN1700509

Product Details anti-SUMO2/3 Antibody (Biotin)

Target: SUMO2/3 (Small Ubiquitin Related Modifier 2/3 (SUMO2/3))

Binding Specificity: AA 21-95

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUMO2/3 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Cow,Horse,Chicken,Rabbit,Zebrafish

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Sumo 2+3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for SUMO2/3

Target: SUMO2/3 (Small Ubiquitin Related Modifier 2/3 (SUMO2/3))

Alternative Name: Sumo 2+3 (SUMO2/3 Products)

Synonyms: SUMO2 antibody, small ubiquitin-like modifier 2 antibody, SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)-like antibody, small ubiquitin-related modifier 2 antibody, SUMO2 antibody, LOC711990 antibody, LOC714892 antibody

Background:

Synonyms: HSMT3, MGC117191, OTTHUMP00000115275, OTTHUMP00000115276, OTTHUMP00000115277, Sentrin 2, Small ubiquitin like modier 2, Small ubiquitin like modier protein 3, Small ubiquitin related modier 2, Small ubiquitin related modier 3, SMT3 A, SMT3 B, SMT3 H1, SMT3 H2, SMT3 homolog 1, SMT3 homolog 2, SMT3 homolog, SMT3 suppressor of m two 3 homolog 1, SMT3 suppressor of m two 3 homolog 2 S. cerevisiae, SMT3 suppressor of m two 3 homolog 2, SMT3 suppressor of m two 3 homolog 3 S. cerevisiae, SMT3 suppressor of m two 3 homolog 3, SMT3A, SMT3B, SMT3H1, SMT3H2, Sumo 2, Sumo 3, Sumo2, Sumo3, Suppressor of m two 3 homolog 2, Suppressor of m two 3 homolog 3, Ubiquitin like protein SMT3A, Ubiquitin like protein SMT3B, SUMO2_HUMAN, SUMO3_HUMAN.

Background: The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, SUMO-2 and SUMO-3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include IkBa, MDM2, p53, PML and Ran GAP1. SUMO-2 and SUMO-3 contribute to a greater percentage of protein modification than does SUMO-1, and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates b-Amyloid generation and may be critical in the onset or progression of Alzheimer?s disease.