IFI30 antibody (AA 181-250) (Biotin)

Catalog No. ABIN1700344

Product Details anti-IFI30 Antibody (Biotin)

Target: IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))

Binding Specificity: AA 181-250

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFI30 antibody is conjugated to Biotin

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human IFI30

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for IFI30

Target: IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))

Alternative Name: I30 (IFI30 Products)

Synonyms: GILT antibody, wu:fb12b04 antibody, zgc:101752 antibody, IP30 antibody, IFI-30 antibody, interferon, gamma-inducible protein 30 antibody, IFI30, lysosomal thiol reductase antibody, interferon gamma inducible protein 30 antibody, ifi30 antibody, IFI30 antibody, LOC100304719 antibody, Ifi30 antibody

Background:

Synonyms: Gamma interferon inducible lysosomal thiol reductase, Gamma interferon inducible protein IP 30, GILT, I 30, interferon gamma inducible protein 30, Interferon gamma inducible protein 30 preproprotein, IP30, Lysosomal thiol reductase, Lysosomal thiol reductase, gamma-interferon-inducible.

Background: Proteins internalized into the endocytic pathway are usually degraded. Efficient proteolysis requires denaturation, induced by acidic conditions within lysosomes, and reduction of inter- and intrachain disulfide bonds. Cytosolic reduction is mediated enzymatically by thioredoxin. In the endocytic pathway, reduction of protein disulfide bonds is important for the generation of MHC class II-peptide complexes. This process is catalyzed by a gamma-interferon-inducible thiol reductase (GILT). GILT is synthesized as a precursor, and following delivery to MHC class II-containing compartments (MIICs), is processed to the mature form via cleavage of amino- and carboxy-terminal propeptides. A lysosomal thiol reductase, GILT, is optimally active at low pH and capable of catalyzing disulfide bond reduction both in vivo and in vitro. GILT is expressed constitutively in antigen-presenting cells and is induced by g-interferon in other cell types, suggesting a potentially important role in antigen processing. Additionally, T cell recognition of select exogenous and endogenous epitopes is dependent on tumor cell expression of GILT. The absence of GILT in melanomas alters antigen processing and the hierarchy of immunodominant epitope presentation.

Gene ID: 10437

UniProt: P13284