MHC, Class I antibody (AA 81-180) (Biotin)

Catalog No. ABIN1700057

Product Details anti-MHC, Class I Antibody (Biotin)

Target: MHC, Class I

Binding Specificity: AA 81-180

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MHC, Class I antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human MHC class I

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for MHC, Class I

Target: MHC, Class I

Alternative Name: MHC class 1 (MHC, Class I Products)

Synonyms: H-2K antibody, H-2K(d) antibody, H2-K antibody, K-f antibody, Y-FA antibody, YFV antibody, histocompatibility 2, K1, K region antibody, MHC class I antigen YF5 antibody, MHC class I antigen antibody, major histocompatibility complex, class I antibody, H2-K1 antibody, YF5 antibody, OVAR antibody, LOC512672 antibody

Background:

Synonyms: A 28, A 9, Aw 24, Aw 68, CLASS I HISTOCOMPATIBILITY ANTIGEN, H2 K1, H2K, HLA A, HLA-A, HLA C, HLA-C, HLA class I histocompatibility antigen A 1 alpha chain, HLA class I histocompatibility antigen A 11 alpha chain, HLA class I histocompatibility antigen A 2 alpha chain, HLA class I histocompatibility antigen A 24 alpha chain, HLA class I histocompatibility antigen A 3 alpha chain, HLA class I histocompatibility antigen A 30 alpha chain, HLA class I histocompatibility antigen A 32 alpha chain, HLA class I histocompatibility antigen A 68 alpha chain, HLAA, Major histocompatibility complex class I A, MHC class I heavy chain H2 K, 1C04_HUMAN.

Background: HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

UniProt: G0ZMJ8