Hephaestin antibody (AA 21-120) (AbBy Fluor® 647)

Catalog No. ABIN1699771

Product Details anti-Hephaestin Antibody (AbBy Fluor® 647)

Target: Hephaestin (HEPH)

Binding Specificity: AA 21-120

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hephaestin antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Hephaestin

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Hephaestin

Target: Hephaestin (HEPH)

Alternative Name: Hephaestin (HEPH Products)

Synonyms: HEPH antibody, C130006F04Rik antibody, Cpl antibody, mKIAA0698 antibody, sla antibody, CPL antibody, hephaestin antibody, HEPH antibody, heph antibody, Heph antibody

Background:

Synonyms: CPL, HEPH, HEPH_HUMAN, Hephaestin.

Background: Hephaestin is a single-pass type I membrane protein that belongs to the multicopper oxidase family of proteins. Hephaestin, a copper-dependant ferroxidase protein, is crucial for iron exiting intestinal enterocytes into the circulation. It mediates the movement of iron across the basolateral membrane in conjunction with ferroportin 1. This is an important link between iron and copper metabolism in mammalian systems, as copper deficiency leads to reduced hephaestin and reduced iron absorption resulting in anemia. Hephaestin can bind six copper ions per monomer and is regulated by the homeobox transcription factor CDX2. Increased levels of iron leads to an increase in CDX2 expression and thus Hephaestin. Hephaestin is primarily detected in the intestine, but is also expressed in colon, breast, bone trabecural cells and fibroblasts.

Gene ID: 9843

Pathways: Transition Metal Ion Homeostasis