CD200 antibody (APC)

Catalog No. ABIN1690745

Product Details anti-CD200 Antibody (APC)

Target: CD200

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD200 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody OX-104 recognizes an extracellular epitope of CD200, a type-1 glycoprotein of the immunoglobulin superfamily, which is expressed in neurons, B and T cell subsets, keratinocytes, follicular dendritic cells, and ovarian cells.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: recombinant human CD200

Clone: OX-104

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD200 antibody (APC) (ABIN1690745)

Colmont, Benketah, Reed, Hawk, Telford, Ohyama, Udey, Yee, Vogel, Patel: "CD200-expressing human basal cell carcinoma cells initiate tumor growth." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 4, pp. 1434-9, (2013) (PubMed).

Li, Zhao, Tong, Qian, Ren, Zhang, Ding, Chen, Wang, Zhang, Zeng, Zhang, Tang, Zhang, Ba, He, Cao, Lipsky: "Aberrant CD200/CD200R1 expression and function in systemic lupus erythematosus contributes to abnormal T-cell responsiveness and dendritic cell activity." in: Arthritis research & therapy, Vol. 14, Issue 3, pp. R123, (2012) (PubMed).

Garza, Yang, Zhao, Blatt, Lee, He, Stanton, Carrasco, Spiegel, Tobias, Cotsarelis: "Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells." in: The Journal of clinical investigation, Vol. 121, Issue 2, pp. 613-22, (2011) (PubMed).

Meuth, Simon, Grimm, Melzer, Herrmann, Spitzer, Landgraf, Wiendl: "CNS inflammation and neuronal degeneration is aggravated by impaired CD200-CD200R-mediated macrophage silencing." in: Journal of neuroimmunology, Vol. 194, Issue 1-2, pp. 62-9, (2008) (PubMed).

Sopp, Werling, Baldwin: "Cross-reactivity of mAbs to human CD antigens with cells from cattle." in: Veterinary immunology and immunopathology, Vol. 119, Issue 1-2, pp. 106-14, (2007) (PubMed).

Shiratori, Yamaguchi, Suzukawa, Yamamoto, Lanier, Saito, Arase: "Down-regulation of basophil function by human CD200 and human herpesvirus-8 CD200." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 7, pp. 4441-9, (2005) (PubMed).

Foster-Cuevas, Wright, Puklavec, Brown, Barclay: "Human herpesvirus 8 K14 protein mimics CD200 in down-regulating macrophage activation through CD200 receptor." in: Journal of virology, Vol. 78, Issue 14, pp. 7667-76, (2004) (PubMed).

Target Details for CD200

Target: CD200

Alternative Name: CD200 (CD200 Products)

Synonyms: MOX1 antibody, MOX2 antibody, MRC antibody, OX-2 antibody, CD200 antibody, Cspmo2 antibody, MRCOX2 antibody, Mox2 antibody, DKFZp459C0716 antibody, OX2 antibody, CD200 molecule antibody, Cd200 molecule antibody, CD200 antigen antibody, CD200 antibody, Cd200 antibody

Background: CD200 Molecule,CD200 (also known as OX2 or MRC) is a type-1 membrane glycoprotein, which contains two extracellular immunoglobulin domains, transmembrane domain and cytoplasmic domain. It is expressed by neuronal cells, B and T cell subsets, follicular dendritic cells, keratinocytes, and ovarian cells. The interaction between CD200 and its receptor CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation along with reduced TNF-alpha and IL-13 secretion and overall attenuation of the activation status of lymphocytes. It seems CD200 is also involved in maternal tolerance and its decreased expression in hair follicle correlates with follicular miniaturization.,MRC, MOX, OX-2

Gene ID: 4345

UniProt: P41217