GIT1 antibody (AA 375-770)

Catalog No. ABIN1686622

Product Details anti-GIT1 Antibody

Target: GIT1

Binding Specificity: AA 375-770

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GIT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~90 kDa. Does not cross-react with GIT2.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 375-770 (C-terminus) of rat GIT1

Clone: S39B-8

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1686622 was sufficient for detection of GIT1 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for GIT1

Target: GIT1

Alternative Name: GIT1 (GIT1 Products)

Synonyms: Cat-1 antibody, p95Cat antibody, GIT ArfGAP 1 antibody, G protein-coupled receptor kinase interacting ArfGAP 1 antibody, G protein-coupled receptor kinase-interactor 1 antibody, GIT1 antibody, Git1 antibody

Background: G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin (reviewed in 1). GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration (2). GIT-1 has also been implicated in neuronal functions including synapse formation (3) and the pathology of Huntington disease (4). Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains (5). Htt interacts directly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology (4).

Gene ID: 83709

NCBI Accession: NP_114002

UniProt: Q9Z272