GABBR2 antibody (AA 861-912)

Catalog No. ABIN1686619

Product Details anti-GABBR2 Antibody

Target: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Binding Specificity: AA 861-912

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GABBR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Detects ~105 kDa. No cross-reactivity against GABA(B)R1.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 861-912 of rat GABA(B)R2

Clone: S81-2

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1686619 was sufficient for detection of GABA(B)R2 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for GABBR2

Target: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Alternative Name: GABA B Receptor 2 (GABBR2 Products)

Synonyms: Gpr51 antibody, GABABR2 antibody, GPR51 antibody, GPRC3B antibody, HG20 antibody, HRIHFB2099 antibody, Gb2 antibody, Gm425 antibody, GABA(B)R2 antibody, si:dkey-190l1.2 antibody, gamma-aminobutyric acid type B receptor subunit 2 antibody, gamma-aminobutyric acid (GABA) B receptor, 2 antibody, gamma-aminobutyric acid (GABA) B receptor, 2 S homeolog antibody, GABBR2 antibody, LOC100448699 antibody, gabbr2 antibody, Gabbr2 antibody, gabbr2.S antibody

Background: GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).

Gene ID: 83633

UniProt: O88871

Pathways: cAMP Metabolic Process