CDC27 antibody (AA 551-830)

Catalog No. ABIN1679113

Product Details anti-CDC27 Antibody

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Binding Specificity: AA 551-830

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Sequence: HLQKDVALSV LSKDLTDMDK NSPEAWCAAG NCFSLQREHD IAIKFFQRAI QVDPNYAYAY TLLGHEFVLT EELDKALACF RNAIRVNPRH YNAWYGLGMI YYKQEKFSLA EMHFQKALDI NPQSSVLLCH IGVVQHALKK SEKALDTLNK AIVIDPKNPL CKFHRASVLF ANEKYKSALQ ELEELKQIVP KESLVYFLIG KVYKKLGQTH LALMNFSWAM DLDPKGANNQ IKEAIDKRYL PDDEEPITQE EQIMGTDESQ ESSMTDADDT QLHAAESDEF

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 551-830 of human CDC27 (NP_001107563.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for CDC27

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Alternative Name: CDC27 (CDC27 Products)

Synonyms: ANAPC3 antibody, APC3 antibody, CDC27Hs antibody, D0S1430E antibody, D17S978E antibody, HNUC antibody, NUC2 antibody, wu:fb54g06 antibody, wu:fc88c12 antibody, AI452358 antibody, BC023187 antibody, CDC27 antibody, NV10210 antibody, cell division cycle 27 antibody, cell division cycle 27 S homeolog antibody, cell division cycle protein 27 homolog antibody, anaphase-promoting complex subunit Apc3 antibody, CDC27 antibody, cdc27 antibody, Cdc27 antibody, cdc27.S antibody, LOC100123129 antibody, nuc2 antibody

Background: The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y.,CDC27,ANAPC3,APC3,CDC27Hs,D0S1430E,D17S978E,H-NUC,HNUC,NUC2,Cell Biology & Developmental Biology,Cell Cycle,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,CDC27

Molecular Weight: 91 kDa/92 kDa

Gene ID: 996

UniProt: P30260