RFP antibody (Biotin)

Catalog No. ABIN1607901

Supplier Product No.: 600-906-379

Product Details anti-RFP Antibody (Biotin)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Chicken

Clonality: Polyclonal

Conjugate: This RFP antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 600-906-379

Supplier: Rockland

Purpose: RFP Antibody Biotin Conjugated

Cross-Reactivity (Details): RFP Antibody was tested by western blot and immunoelectrophoresis against anti-biotin, anti-chicken serum, and RFP.

Characteristics: Synonyms: chicken anti-RFP antibody biotin conjugation, biotin conjugated chicken anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Purification: RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Application Note: Anti-RFP is designed to detect recombinant RFP. This antibody has been tested to detect RFP by immunoblotting and immunofluorescence and is suitable for use in immunohistochemistry. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.

Western Blot Dilution: 1:1,000 - 1:3,000

ELISA Dilution: 1:10,000

IF Microscopy Dilution: 1:100

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-RFP antibody (Biotin) (ABIN1607901)

Jo, Xu, Deniz, Cherian, DeVries, Zhu: "Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina." in: Frontiers in neural circuits, Vol. 12, pp. 66, (2019) (PubMed).

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: DsRed (RFP Products)

Background: Background: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including western blotting.

UniProt: Q9U6Y8