RFP antibody (Biotin)

Catalog No. ABIN1607901

Product Details anti-RFP Antibody (Biotin)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Chicken

Clonality: Polyclonal

Conjugate: This RFP antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Purpose: Anti-RFP is designed to detect recombinant RFP.

Purification: RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by ELISA, western blot and immunoelectrophoresis.

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Immunogen Type: Recombinant Protein

Application Details

Application Notes: Anti-RFP is designed to detect recombinant RFP. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
ELISA Dilution: 1:10.000
Western Blot Dilution: 1:1.000 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with 100 µL deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: Expiration date is one (1) year from date of opening.

References for anti-RFP antibody (Biotin) (ABIN1607901)

Jo, Xu, Deniz, Cherian, DeVries, Zhu: "Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina." in: Frontiers in neural circuits, Vol. 12, pp. 66, (2019) (PubMed).

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Abstract: RFP Products

Background: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS). Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Gene ID: 55976617

UniProt: Q9U6Y8