Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)
Cross-Reactivity (Details)
Cross reactivity with PIP5K2B from other sources has not been determined.
Purity
Anti-PIP5K2B was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards PIP5K2B. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat, Chicken, and Primate based on 100 % sequence homology.
Endotoxin Level
Low Endotoxin : No
Immunogen
PIP5K2B affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human PIP5K2B. Immunogen Type: Peptide
Anti-PIP5K2B antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~54 kDa corresponding to the appropriate cell lysate or extract. ELISA Dilution: 1:20.000 - 1:60.000 Immunohistochemistry Dilution: 1:100-1:500 IF Microscopy Dilution: 1:100-1:500 Western Blot Dilution: 0.2 µg/mL
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Storage
4 °C/-20 °C
Storage Comment
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date
Expiration date is six (6) months from date of opening.
Target
PIP4K2B
(Phosphatidylinositol-5-Phosphate 4-Kinase, Type II, beta (PIP4K2B))
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Background
PIP5K2B (phosphatidylinositol-4-phosphate 5-kinase, type II, beta) functions in the Pkc1p protein kinase pathway and is required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization. Anti-PIP5K2B antibodies are ideal for researchers interested in Cancer research. Synonyms: 1-phosphatidylinositol-4-phosphate kinase, 1-phosphatidylinositol-5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, EC 2.7.1, EC 2.7.1.149, phosphatidylinositol-4-phosphate 5-kinase, type II, beta, Phosphatidylinositol-5-phosphate 4-kinase type II beta, phosphatidylinositol-5-phosphate 4-kinase type-2 beta