PARL antibody (C-Term)

Catalog No. ABIN1607894

Supplier Product No.: 600-401-j27

Product Details anti-PARL Antibody

Target: PARL (Presenilin Associated, Rhomboid-Like (PARL))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Chicken, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-j27

Supplier: Rockland

Purpose: PARL Antibody

Cross-Reactivity (Details): This antibody is specific towards PARL.

Characteristics: Synonyms: rabbit anti-PARL antibody, p-beta, Pbeta, presenilin associated rhomboid-like protein mitochondrial, PRO2207, Mitochondrial intramembrane cleaving protease PARL, PSARL

Purification: Anti-PARL was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: PARL affinity purified antibody was prepared from whole rabbit serum produced by repeated immunization from a synthetic peptide corresponding to the C terminus region of human PARL.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-PARL antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~42.2 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:20,000 - 1:60,000

IF Microscopy Dilution: 1:100-1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.86 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for PARL

Target: PARL (Presenilin Associated, Rhomboid-Like (PARL))

Alternative Name: PARL (PARL Products)

Background: Background: PARL (presenilin associated, rhomboid-like) encodes a mitochondrial integral membrane protein. Following proteolytic processing of this protein, a small peptide (P-beta) is formed and translocated to the nucleus. This gene may be involved in signal transduction via regulated intramembrane proteolysis of membrane-tethered precursor proteins. Variation in this gene has been associated with increased risk for type 2 diabetes. Alternative splicing results in multiple transcript variants encoding different isoforms. Anti-PARL antibodies are ideal for researchers interested in Apoptosis, Alzheimer's Research, Neuroscience, and Mitochondrial Fusion Proteins research.

Gene ID: 55486

NCBI Accession: NP_061092

UniProt: Q9H300

Pathways: Autophagy