KMO antibody (AA 300-400)

Catalog No. ABIN1607892

Supplier Product No.: 600-401-j25

Product Details anti-KMO Antibody

Target: KMO (Kynurenine 3-Monooxygenase (Kynurenine 3-Hydroxylase) (KMO))

Binding Specificity: AA 300-400

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KMO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-j25

Supplier: Rockland

Purpose: Kmo Antibody

Cross-Reactivity (Details): This antibody is specific towards Kynurenine 3-monooxygenase (KMO).

Characteristics: Synonyms: rabbit anti-KMO antibody, kynurenine 3-monooxygenase, kynurenine 3-hydroxylase

Purification: Anti-KMO was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: KMO affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding 300-400aa of the mouse KMO chain.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-KMO antibody is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~58 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:20,000 - 1:60,000

IF Microscopy Dilution: 1:100-1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.08 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for KMO

Target: KMO (Kynurenine 3-Monooxygenase (Kynurenine 3-Hydroxylase) (KMO))

Alternative Name: KMO (KMO Products)

Background: Background: Kynurenine 3-monooxygenase (KMO) catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). The enzyme is required for synthesis of quinolinic acid. Quinolinic acid is a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Anti-KMO antibodies are ideal for researchers interested in Apoptosis, Neurodegeneration, and Neuroscience research.

Gene ID: 8564

NCBI Accession: NP_003670

UniProt: O15229