HDAC7 antibody (N-Term)

Catalog No. ABIN1607889

Product Details anti-HDAC7 Antibody

Target: HDAC7 (Histone Deacetylase 7 (HDAC7))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Cross-Reactivity (Details): Cross reactivity with HDAC7 from other sources has not been determined.

Purity: Anti-HDAC7 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards HDAC7. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat and Primate based on 100 % sequence homology.

Endotoxin Level: Low Endotoxin : No

Immunogen: HDAC7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N terminal region of human HDAC7.
Immunogen Type: Peptide

Application Details

Application Notes: Anti-HDAC7 antibody is useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~103kDa and isoforms at ~70, and 40 kDa corresponding to the appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:60.000
Immunohistochemistry Dilution: 1:100-1:500
IF Microscopy Dilution: 1:100-1:500
Western Blot Dilution: 1 µg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.55 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: Expiration date is six (6) months from date of opening.

Target Details for HDAC7

Target: HDAC7 (Histone Deacetylase 7 (HDAC7))

Alternative Name: HDAC7 (HDAC7 Products)

Synonyms: HD7A antibody, HDAC7A antibody, 5830434K02Rik antibody, HD7 antibody, HD7a antibody, Hdac7a antibody, mFLJ00062 antibody, hdac7a antibody, K2K18.5 antibody, K2K18_5 antibody, histone deacetylase7 antibody, histone deacetylase 7 antibody, histone deacetylase7 antibody, HDAC7 antibody, Hdac7 antibody, hdac7 antibody, HDA7 antibody

Background: HDAC7 is a member of the class II mammalian histone deacetylases, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional corepressor complexes that can be recruited to specific promoter regions via interactions with a growing number of sequence specific transcriptional factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription. Anti-HDAC7 antibodies are ideal for researches interested in Breast Cancer, Cancer, Cell Cycle and Replication, Chromatin Research, Epigenetics, and Histone Deacetylases research. Synonyms: DKFZP586J0917, EC 3.5.1.98, HD7, HD7A, HD7a, HDAC7AFLJ99588, histone deacetylase 7, Histone deacetylase 7ADKFZp586J0917

Gene ID: 51564

NCBI Accession: NP_001091886

UniProt: Q8WUI4

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development