ARHGAP22 antibody (pSer22)

Catalog No. ABIN1607878

Supplier Product No.: 600-401-j11

Product Details anti-ARHGAP22 Antibody

Target: ARHGAP22 (rho GTPase Activating Protein 22 (ARHGAP22))

Binding Specificity: pSer22

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGAP22 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-j11

Supplier: Rockland

Purpose: Arhgap22 phospho S22 Antibody

Cross-Reactivity (Details): This antibody is specific for phosphorylated ARHGAP22 at Serine 22.

Characteristics: Synonyms: rabbit anti-ARHGAP22 pS22 Antibody, Rho-type GTPase-activating protein 22, RHOGAP2, Rho GTPase activating protein 22, rho GTPase-activating protein 22, ARHGAP 22, ARHGAP-22

Purification: Anti-ARHGAP22 pS22 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide corresponding to the region surrounding mouse pS22 region of ARHGAP22.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-ARHGAP22 pS22 antibody is tested in Western Blot and ELISA useful for Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77.8 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:20,000 - 1:60,000

IF Microscopy Dilution: 1:100-1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.07 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for ARHGAP22

Target: ARHGAP22 (rho GTPase Activating Protein 22 (ARHGAP22))

Alternative Name: Arhgap22 (ARHGAP22 Products)

Background: Background: ARHGAP22 is a Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. It acts as a GTPase activator for RAC1 by converting it to an inactive GDP-bound state and also inhibits RAC1-dependent lamellipodia formation. It may also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter. Anti-ARHGAP22 [p Ser22] antibody is ideal for researchers interested in Diabetes Research, Lipid and Metabolism research.

Gene ID: 239027

NCBI Accession: NP_722495

UniProt: Q8BL80