ARHGAP22 antibody (phosphorylated)

Catalog No. ABIN1607878

Product Details anti-ARHGAP22 Antibody

Target: ARHGAP22 (rho GTPase Activating Protein 22 (ARHGAP22))

Binding Specificity: phosphorylated

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGAP22 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Cross-Reactivity (Details): Cross reactivity with ARHGAP22 pS22 from other sources has not been determined.

Purity: Anti-ARHGAP22 pS22 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for phosphorylated ARHGAP22 at Serine 22. It also recognizes the S397-> A mutation but not the S22-> A mutation. A BLAST analysis was used to suggest cross-reactivity with Mouse, Rat and Human based on 100 % sequence homology.

Endotoxin Level: Low Endotoxin : No

Immunogen: ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the region surrounding mouse pS22 region of ARHGAP22.
Immunogen Type: Peptide

Application Details

Application Notes: Anti-ARHGAP22 pS22 antibody is useful for ELISA, Western Blot and Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77.8 kDa corresponding to the appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:60.000
Immunohistochemistry Dilution: 1:100-1:500
IF Microscopy Dilution: 1:100-1:500
Western Blot Dilution: 1 µg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.07 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: Expiration date is six (6) months from date of opening.

Target Details for ARHGAP22

Target: ARHGAP22 (rho GTPase Activating Protein 22 (ARHGAP22))

Alternative Name: ARHGAP22 (ARHGAP22 Products)

Synonyms: si:ch211-223a10.2 antibody, RhoGAP2 antibody, RhoGap22 antibody, B230341L19Rik antibody, RHOGAP2 antibody, p68RacGAP antibody, Rho GTPase activating protein 22 antibody, rho GTPase activating protein 22 antibody, ARHGAP22 antibody, arhgap22 antibody, CC1G_06014 antibody, Arhgap22 antibody

Background: ARHGAP22 is a Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. It acts as a GTPase activator for RAC1 by converting it to an inactive GDP-bound state and also inhibits RAC1-dependent lamellipodia formation. It may also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter. Anti-ARHGAP22 [p Ser22] antibody is ideal for researchers interested in Diabetes Research, Lipid and Metabolism research. Synonyms: Rho-type GTPase-activating protein 22, RHOGAP2, RhoGAP2, Rho GTPase activating protein 22, rho GTPase-activating protein 22

Gene ID: 239027

NCBI Accession: NP_722495

UniProt: Q8BL80