Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
Cross-Reactivity (Details)
Cross reactivity with Abeta from other sources has not been determined.
Purity
Anti-Beta Amyloid 40 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific to Beta Amyloid 40 (Abeta 40) and will not react with the ABeta 42 form. A BLAST analysis was used to suggest cross-reactivity with Human, Primate, Rat, and Mouse based on 100 % sequence homology.
Endotoxin Level
Low Endotoxin : No
Immunogen
Beta Amyloid 40 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human Abeta. Immunogen Type: Peptide
Abeta 1-40
Reactivity: Human, Rat, Mouse, Pig, Chicken, Cow, Dog
IF (p)
Host: Rabbit
Polyclonal
AbBy Fluor® 488
Application Notes
Anti-Beta Amyloid 40 antibody is useful for Western Blot and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~86.9 kDa corresponding to the appropriate cell lysate or extract. ELISA Dilution: 1:20.000 - 1:60.000 Immunohistochemistry Dilution: 1:100-1:500 IF Microscopy Dilution: 1:100-1:500 Western Blot Dilution: 1 µg/mL
Restrictions
For Research Use only
Format
Liquid
Concentration
1.12 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 50 % (v/v) Glycerol
Storage
4 °C/-20 °C
Storage Comment
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date
Expiration date is six (6) months from date of opening.
Beta Amyloid 40 protein (Abeta) is associated with neuronal injury and death in Alzheimer's disease. These proteins are the main components of amyloid plaques. Anti-Beta Amyloid 40 protein (Abeta) antibody is ideal for researchers interested in Apoptosis, Alzheimers Research, Cell Cycle and Replication, Neurodegeneration, Neuroscience, and Neuronal Cell Marker reserach. Synonyms: amyloid beta 40, Beta-amyloid protein 40, Abeta, ß Amyloid Antibody