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CX3CL1 antibody (Biotin)

CX3CL1 Reactivity: Human WB, ELISA Host: Rabbit Polyclonal Biotin
Catalog No. ABIN1607866
  • Target See all CX3CL1 Antibodies
    CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
    Reactivity
    • 74
    • 36
    • 31
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 72
    • 8
    • 3
    • 2
    • 1
    Rabbit
    Clonality
    • 76
    • 10
    Polyclonal
    Conjugate
    • 46
    • 8
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CX3CL1 antibody is conjugated to Biotin
    Application
    • 70
    • 25
    • 23
    • 20
    • 13
    • 13
    • 7
    • 7
    • 7
    • 7
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Cross-Reactivity (Details)
    Cross reactivity with Fractalkine from other sources has not been determined.
    Purity
    Anti-Fractalkine is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human Fractalkine protein. A BLAST analysis was used to suggest cross-reactivity with Fractalkine from human sources based on 100 % homology with the immunizing sequence. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum.
    Endotoxin Level
    Low Endotoxin : No
    Immunogen
    Anti-Fractalkine antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human Fractalkine protein from E. coli.
    Immunogen Type: Recombinant Protein
    Isotype
    IgG
    Top Product
    Discover our top product CX3CL1 Primary Antibody
  • Application Notes
    Fractalkine purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~42 kDa in size corresponding to the human Fractalkine protein by western blotting in appropriate cell lysate or extract.
    ELISA Dilution: 1:20.000 - 1:50.000
    Fluorochrome Protein Value: 10-20
    Western Blot Dilution: 1:1000-1:2000
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Restore with 100 µL deionized water (or equivalent)
    Concentration
    1 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    Expiration date is one (1) year from date of opening.
  • Target
    CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
    Alternative Name
    Fractalkine (CX3CL1 Products)
    Synonyms
    ABCD3 antibody, CX3CL1 antibody, DKFZp459K117 antibody, Cx3c antibody, Scyd1 antibody, ABCD-3 antibody, C3Xkine antibody, CXC3 antibody, CXC3C antibody, NTN antibody, NTT antibody, SCYD1 antibody, fractalkine antibody, neurotactin antibody, AB030188 antibody, AI848747 antibody, CX3C antibody, Cxc3 antibody, D8Bwg0439e antibody, ATP binding cassette subfamily D member 3 antibody, C-X3-C motif chemokine ligand 1 antibody, chemokine (C-X3-C motif) ligand 1 antibody, ABCD3 antibody, CX3CL1 antibody, Cx3cl1 antibody, Abcd3 antibody
    Background
    Fractalkine (CX3CL1) is an atypical chemokine that was the first of a fourth chemokine motif (CX3C). It is thought to function as a T cell and monocyte chemotractant and is produced by non-haemopoietic cells. Fractalkine is made in a soluble and membrane bound form in activated endothelial cells which is thought to promote adhesion of leukocytes. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. It is found predominantly in the small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas. Fractalkine may play a role in regulating leukocyte adhesion and migration processes at the endothelium by binding to CX3CR1. Recombinant human Fractalkine is a non-glycosylated protein with a molecular weight of 8.6 kDa. Anti-Fractalkine antibody is ideal for researchers interested in cytokines and stem cell research. Synonyms: CX3CL1, Fractalkine, Chemokine (C-X3-C motif) ligand 1, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1, FKN, NTT, SCYD1, A-152E5.2
    Gene ID
    6376
    NCBI Accession
    NP_002987
    UniProt
    P78423
    Pathways
    Synaptic Membrane
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