Thymic Stromal Lymphopoietin antibody (Biotin)

Catalog No. ABIN1607864

Supplier Product No.: 209-406-h48

Product Details anti-Thymic Stromal Lymphopoietin Antibody (Biotin)

Target: Thymic Stromal Lymphopoietin (TSLP)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Thymic Stromal Lymphopoietin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Supplier Product No.: 209-406-h48

Supplier: Rockland

Purpose: TSLP Antibody Biotin Conjugated

Cross-Reactivity (Details): This antibody is specific for human TSLP protein.

Characteristics: Synonyms: rabbit anti-TSLP biotin conjugated antibody, rabbit anti-Thymic Stromal Lymphopoietin biotin conjugated antibody

Purification: Anti-TSLP is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-TSLP antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human TSLP protein from E. coli.

Immunogen Type: Recombinant Protein

Isotype: IgG

Labeling Ratio: 10-20

Application Details

Application Notes:

Application Note: TSLP purified antibody has been tested in western blotting and suitable in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kDa in size corresponding to the human TSLP protein by western blotting in appropriate cell lysate or extract.

Western Blot Dilution: 1:1000-1:2000

ELISA Dilution: 1:20,000 - 1:50,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Thymic Stromal Lymphopoietin

Target: Thymic Stromal Lymphopoietin (TSLP)

Alternative Name: TSLP (TSLP Products)

Background: Background: Thymic Stromal Lymphopoietin (TSLP) is a hematopoietic cytokine. TSLP is mainly expressed in the heart, liver and prostate. TSLP sends signals through a heterodimeric receptor complex, containing the TSLP-R and the IL-7Rα chain to induce the release of T cell-attracting chemokines from monocytes. It can induce allergic inflammation by directly activating mast cells. It also promotes T helper type 2 (TH2) cell responses associated with immunity, including atopic dermatitis and asthma, and can promote TH2 cytokine-associated inflammation by directly promoting the effector functions of CD4+ TH2 cells, basophils and other granulocyte populations while simultaneously limiting the expression of dendritic cells derived proinflammatory cytokines and promoting regulatory T cell responses in peripheral tissues. Recombinant human TSLP is a non-glycosylated protein with a molecular weight of 15 kDa. Anti-TSLP antibody is ideal for researchers interested in cytokines and stem cell research.

Gene ID: 85480

NCBI Accession: NP_149024

UniProt: Q969D9