Thymic Stromal Lymphopoietin antibody

Catalog No. ABIN1607858

Product Details anti-Thymic Stromal Lymphopoietin Antibody

Target: Thymic Stromal Lymphopoietin (TSLP)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Thymic Stromal Lymphopoietin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity with TSLP from other sources has not been determined.

Purity: Anti-TSLP is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human TSLP protein. A BLAST analysis was used to suggest cross-reactivity with TSLP from human sources based on 100 % homology with the immunizing sequence.

Endotoxin Level: Low Endotoxin : No

Immunogen: Anti-TSLP antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human TSLP protein from E. coli.
Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes: TSLP purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18 kDa in size corresponding to the human TSLP protein by western blotting in appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:50.000
Western Blot Dilution: 1:1000-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Thymic Stromal Lymphopoietin

Target: Thymic Stromal Lymphopoietin (TSLP)

Alternative Name: TSLP (TSLP Products)

Synonyms: TSLP antibody, thymic stromal lymphopoietin antibody, TSLP antibody, Tslp antibody

Background: Thymic Stromal Lymphopoietin (TSLP) is a hematopoietic cytokine. TSLP is mainly expressed in the heart, liver and prostate. TSLP sends signals through a heterodimeric receptor complex, containing the TSLP-R and the IL-7Rα chain to induce the release of T cell-attracting chemokines from monocytes. It can induce allergic inflammation by directly activating mast cells. It also promotes T helper type 2 (TH2) cell responses associated with immunity, including atopic dermatitis and asthma, and can promote TH2 cytokine-associated inflammation by directly promoting the effector functions of CD4+ TH2 cells, basophils and other granulocyte populations while simultaneously limiting the expression of dendritic cells derived proinflammatory cytokines and promoting regulatory T cell responses in peripheral tissues. Recombinant human TSLP is a non-glycosylated protein with a molecular weight of 15 kDa. Anti-TSLP antibody is ideal for researchers interested in cytokines and stem cell research. Synonyms: Thymic Stromal Lymphopoietin

Gene ID: 85480

NCBI Accession: NP_149024

UniProt: Q969D9