Albumin antibody (Biotin)

Catalog No. ABIN1607854

Supplier Product No.: 201-4633-0100

Product Details anti-Albumin Antibody (Biotin)

Target: Albumin (ALB)

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Albumin antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Supplier Product No.: 201-4633-0100

Supplier: Rockland

Purpose: Bovine Serum Albumin Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Albumin (Bovine Serum].

Characteristics: Synonyms: Allergen Bos d 6 antibody, BSA antibody, DKFZp779N1935 antibody, rabbit anti-bovine albumin biotin conjugation, ALB antibody, Albumin antibody

Purification: Bovine serum antibody was purified as an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Albumin (Bovine Serum)

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:250 - 1:1,250

Application Note: Anti-BSA Biotin Conjugated antibody has been tested by ELISA and western blot for detection of bovine serum albumin in biological samples and is suitable in immunohistochemistry assays. Specific dilutions are recommended but should be optimized by researcher.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Albumin

Target: Albumin (ALB)

Alternative Name: ALB (ALB Products)

Background: Background: Anti-BSA antibody is specific for the full-length BSA precursor protein is 607 amino acids in length. An N-terminal 18-residue signal peptide is cut off from the precursor protein upon secretion, and an additional 4 amino acids is cleaved to yield the mature BSA protein that contains 585 amino acids. BSA has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry, and it is also used as a nutrient in cell and microbial culture. BSA is used as a stabilizer for some proteins and enzymes. This protein does not affect other enzymes that do not need it for stabilization.

Gene ID: 280717

UniProt: P02769

Pathways: Lipid Metabolism