ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Cross-Reactivity (Details)
Cross reactivity against Albumin from other tissues and species may occur but has not been specifically determined.
Purification
Bovine Serum Albumin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Albumin (Bovine Serum).
Endotoxin Level
Low Endotoxin : No
Immunogen
Anti-Bovine Serum Albumin antibody was produced by repeated immunizations with bovine serum albumin. Immunogen Type: Native Protein
Anti-ALB antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. ELISA Dilution: 1:20.000 - 1:100.000 Immunohistochemistry Dilution: 1:1.000 - 1:5.000 Western Blot Dilution: 1:2.000-1:10.000
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Restore with 100 µL deionized water (or equivalent)
Concentration
1 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Storage
4 °C/-20 °C
Storage Comment
Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date
Expiration date is one (1) year from date of opening.
PRO0883 antibody, PRO0903 antibody, PRO1341 antibody, albumin antibody, ALB antibody
Background
Bovine Serum Albumin antibody detects BSA. Serum albumin is the main protein of plasma. It has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. ALB is the major zinc transporter in plasma, it typically binds about 80 % of all plasma zinc. BSA antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research. Synonyms: ALB antibody, Albumin antibody, Allergen Bos d 6 antibody, BSA antibody, DKFZp779N1935 antibody, Anti-BSA