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LPO antibody (Biotin)

Rockland 200-606-146-0100 LPO Reactivity: Cow WB, IP, ELISA, DB Host: Sheep Polyclonal Biotin
Rockland
Catalog No. ABIN1607841
Supplier Product No.: 200-606-146-0100
  • Target See all LPO Antibodies
    LPO (Lactoperoxidase (LPO))
    Reactivity
    • 11
    • 7
    • 6
    • 2
    • 2
    • 2
    • 1
    Cow
    Host
    • 16
    • 3
    • 2
    Sheep
    Clonality
    • 19
    • 2
    Polyclonal
    Conjugate
    • 12
    • 7
    • 2
    This LPO antibody is conjugated to Biotin
    Application
    • 17
    • 12
    • 10
    • 8
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), ELISA, Dot Blot (DB)
    Supplier Product No.
    200-606-146-0100
    Supplier
    Rockland
    Purpose
    Anti-Lactoperoxidase Antibody Biotin Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Lactoperoxidase [Bovine Milk].
    Characteristics
    Synonyms: sheep anti-Lactoperoxidase biotin conjugated Antibody, biotin conjugated sheep anti-Lactoperoxidase Antibody, LPO antibody, Salivary peroxidase antibody, SAPX antibody, SPO antibody
    Purification
    Anti-Lactoperoxidase (Bovine Milk) (Sheep) Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen

    Immunogen: Lactoperoxidase [Bovine Milk]

    Immunogen Type: Native Protein

    Isotype
    IgG
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    Discover our top product LPO Primary Antibody
  • Application Notes

    Application Note: Anti-Lactoperoxidase Biotin Conjugated Antibody has been tested by Dot blot and western blot and is suitable to be assayed against 1.0 μg of Lactoperoxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for Anti-Lactoperoxidase.

    Western Blot Dilution: 1:50 - 1:500

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:500 - 1:2,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    10 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    LPO (Lactoperoxidase (LPO))
    Alternative Name
    Lactoperoxidase (LPO Products)
    Background
    Background: Lactoperoxidase antibody recognizes the lactoperoxidase protein. Lactoperoxidase catalyzes the oxidation of a number of inorganic and organic substrates by hydrogen peroxide. Lactoperoxidase plays an important role in killing bacteria in milk. Lactoperoxidase conjugated to Biotin is used to detect the specific target of the lactoperoxidase protein. Lactoperoxidase conjugated to Biotin is suitable for researchers in immunology and biochemistry.
    Gene ID
    280844
    NCBI Accession
    NP_776358
    UniProt
    P80025
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