Malate Dehydrogenase (MDH) antibody (Biotin)

Catalog No. ABIN1607839

Product Details

Target: Malate Dehydrogenase (MDH)

Reactivity: Pig

Host: Sheep

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Malate Dehydrogenase from other sources is unknown.

Purity: Anti-Malate dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].

Endotoxin Level: Low Endotoxin : No

Immunogen: Malate Dehydrogenase [Pig Heart]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Malate dehydrogenase antibody has been assayed against 1.0 µg of Malate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:11.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Malate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:11.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details

Target: Malate Dehydrogenase (MDH)

Alternative Name: Malate Dehydrogenase (MDH Products)

Synonyms: CG5889 antibody, Dmel\\CG5889 antibody, MDH antibody, Mdh antibody, anon-EST:Posey79 antibody, MALATE DEHYDROGENASE antibody, malate dehydrogenase antibody, An15g00070 antibody, AO090701000013 antibody, DDBDRAFT_0188779 antibody, DDBDRAFT_0230186 antibody, DDB_0188779 antibody, DDB_0230186 antibody, Malic enzyme b antibody, oxidoreductase, putative antibody, malate dehydrogenase antibody, NAD dependent malate dehydrogenase Mdh antibody, Men-b antibody, malate dehydrogenase antibody, MDH antibody, mdh antibody, APH_RS02635 antibody, ANI_1_12134 antibody, AOR_1_26114 antibody, PMI_RS16925 antibody, mdhA antibody, mdh1 antibody

Background: Anti-Malate dehydrogenase recognizes the protein malate dehydrogenase, an oxidoreductase that catalyzes the oxidation of malate to oxaloacetate with the concomitant reduction of NAD+ to NADH. This reaction occurs in various metabolic pathways, the most notable being the citric acid cycle and gluconeogenesis. Two isoforms of malate dehyrogenase exist in eukaryotes localized in either the cytoplasm or the mitochondrial matrix. Synonyms: Malate dehydrogenase, cytoplasmic EC=1.1.1.37 Cytosolic malate dehydrogenase

Gene ID: 396894

UniProt: P11708

Pathways: Regulation of Lipid Metabolism by PPARalpha