Aspartate Transaminase antibody (Biotin)

Catalog No. ABIN1607837

Product Details anti-Aspartate Transaminase Antibody (Biotin)

Target: Aspartate Transaminase (Ast)

Reactivity: Pig

Host: Sheep

Clonality: Polyclonal

Conjugate: This Aspartate Transaminase antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB)

Cross-Reactivity (Details): Cross reactivity against Aspartate Aminotransferase (AST) from other sources may occur but has not been specifically determined.

Purity: Anti-Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart].

Endotoxin Level: Low Endotoxin : No

Immunogen: Aspartate Aminotransferase (AST) [Pig Heart]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Aspartate Transaminase has been assayed against 1.0 µg of Aspartate Aminotransferase (AST in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Aspartate Aminotransferase (AST in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Aspartate Transaminase

Target: Aspartate Transaminase (Ast)

Alternative Name: Aspartate Transaminase (Ast Products)

Synonyms: Afu6g02490 antibody, Afu2g09650 antibody, aspartate transaminase antibody, Aspartate transaminase antibody, Aspartate transaminase, putative antibody, aspB antibody, CNM00360 antibody, AFUA_6G02490 antibody, AFUA_2G09650 antibody, AAT_1 antibody, Dd586_1627 antibody, aspB3 antibody, Fbal_1917 antibody, Emin_0749 antibody, CGB_M0340W antibody, Sgly_0435 antibody, Weevi_1216 antibody, Selsp_1595 antibody, Trebr_2143 antibody

Background: Anti-Aspartate Transaminase antibody is present in the cytoplasm of cells, belonging to the class-I pyridoxal-phosphate-dependent aminotransferase family. Playing an active role in amino acid metabolism, Anti-Aspartate Transaminase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction research. Synonyms: Aspartate aminotransferase 1 antibody, Aspartate aminotransferase cytoplasmic antibody, Aspartate aminotransferase cytosolic antibody, ec 2.6.1.1 antibody, GIG 18 antibody, GIG18 antibody, Glutamate oxaloacetate transaminase 1 antibody

Gene ID: 396967

UniProt: P00503