Trypsin Inhibitor antibody (Biotin)
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- Target See all Trypsin Inhibitor products
- Trypsin Inhibitor
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Reactivity
- Lima bean
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Trypsin Inhibitor antibody is conjugated to Biotin
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Application
- Western Blotting (WB), ELISA, Dot Blot (DB)
- Supplier Product No.
- 200-4687-0100
- Supplier
- Rockland
- Purpose
- Trypsin Inhibitor Antibody Biotin Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Lima Bean].
- Characteristics
- Synonyms: rabbit anti-trypsin inhibitor antibody biotin conjugation, biotin conjugated rabbit anti-trypsin inhibitor antibody, 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody
- Purification
- Anti-Trypsin Inhibitor antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Immunogen
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Immunogen: Trypsin Inhibitor [Lima Bean]
Immunogen Type: Native Protein
- Isotype
- IgG
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- Application Notes
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Application Note: Anti-Trypsin Inhibitor antibody has been tested by dot blot and assayed against 1.0 μg of Trypsin Inhibitor in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:6,000 to 1:26,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:250
ELISA Dilution: 1:500 - 1:2,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 10 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Trypsin Inhibitor
- Abstract
- Trypsin Inhibitor Products
- Background
- Background: Soy Bean Trypsin Inhibitor is a serine protease inhibitor, an inhibitor of trypsin, therefore preventing the further breakdown of proteins.
- Gene ID
- 547831
- UniProt
- P01070
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