XDH antibody (Biotin)

Catalog No. ABIN1607817

Supplier Product No.: 200-4683-0100

Product Details anti-XDH Antibody (Biotin)

Target: XDH (Xanthine Dehydrogenase (XDH))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XDH antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Supplier Product No.: 200-4683-0100

Supplier: Rockland

Purpose: Xanthine Oxidase Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk].

Characteristics: Synonyms: rabbit anti-Xanthine Oxidase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Xanthine Oxidase Antibody, Xanthine dehydrogenase antibody, Xanthine dehydrogenase/oxidase antibody, Xanthine oxidase antibody, Xanthine oxidoreductase antibody, xdh antibody, xdha antibody, XO antibody, xor antibody

Purification: Anti-Xanthine Oxidase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Xanthine Oxidase [Bovine Buttermilk]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Xanthine Oxidase Biotin conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Xanthine Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:250

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:500 - 1:2,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for XDH

Target: XDH (Xanthine Dehydrogenase (XDH))

Alternative Name: XDH (XDH Products)

Background: Background: Anti-Xanthine Oxidase recognizes xanthine oxidase, an oxidoreductase. Xanthine oxidase catalyzes the oxidation of hypoxanthine to xanthine and xanthine to uric acid. Both of these reactions also generate the reactive oxygen species hydrogen peroxide. In addition, xanthine oxidase is involved in the catabolism of purines and metabolism of caffeine. Clinically, xanthine oxidase has been linked to gout, a condition in which uric acid accumulates in the kidneys and joints. Anti-Xanthine Oxidase antibody is ideal for investigators interested in Metabolism and Signal Transduction research.

Gene ID: 280960

UniProt: P80457

Pathways: Positive Regulation of Endopeptidase Activity