Trypsinogen antibody (Biotin)
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- Target See all Trypsinogen products
- Trypsinogen
- Reactivity
- Cow
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Trypsinogen antibody is conjugated to Biotin
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Application
- Western Blotting (WB), ELISA, Dot Blot (DB)
- Supplier Product No.
- 200-4680-0100
- Supplier
- Rockland
- Purpose
- Trypsinogen Antibody Biotin Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsinogen [Bovine Pancreas].
- Characteristics
- Synonyms: rabbit anti-Trypsinogen Antibody biotin Conjugation, biotin Conjugated rabbit anti-Trypsinogen Antibody, Brain trypsinogen antibody, Cationic trypsinogen antibody, Mesotrypsinogen antibody, Pancreatic anionic trypsinogen antibody, Protease, serine, 2 antibody, PRSS2 antibody
- Purification
- Anti-Trypsinogen antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Immunogen
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Immunogen: Trypsinogen [Bovine Pancreas]
Immunogen Type: Native Protein
- Isotype
- IgG
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anti-Trypsinogen antibody (HRP)
Reactivity: Cow WB, ELISA Host: Rabbit Polyclonal HRP
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- Application Notes
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Application Note: Anti-Trypsinogen Biotin Conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Trypsinogen in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 10 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Trypsinogen
- Abstract
- Trypsinogen Products
- Background
- Background: Trypsinogen is the precursor form or zymogen of the pancreatic enzyme trypsin. It is found in pancreatic juice, along with amylase, lipase, and chymotrypsinogen. It is activated by enteropeptidase, which is found in the intestinal mucosa, to form trypsin. Once activated, the trypsin can activate more trypsinogen into trypsin. Trypsin cleaves the peptide bond on the carboxyl side of basic amino acids such as arginine and lysine. Anti-Trypsinogen antibody is ideal investigator involved in cytokines and growth factor research.
- Gene ID
- 282603
- UniProt
- Q29463
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