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Penicillinase antibody (Biotin)

Reactivity: Enterobacter cloacae WB, IP, ELISA Host: Rabbit Polyclonal Biotin
Catalog No. ABIN1607809
  • Target See all Penicillinase products
    Penicillinase
    Reactivity
    • 4
    • 1
    Enterobacter cloacae
    Host
    • 4
    • 1
    Rabbit
    Clonality
    • 4
    • 1
    Polyclonal
    Conjugate
    • 4
    • 1
    This Penicillinase antibody is conjugated to Biotin
    Application
    • 5
    • 4
    • 3
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), ELISA
    Cross-Reactivity (Details)
    Cross reactivity against Penicillinase from other sources is unknown.
    Purity
    Anti-Penicillinase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae].
    Endotoxin Level
    Low Endotoxin : No
    Immunogen
    Penicillinase [Enterobacter cloacae]
    Immunogen Type: Native Protein
    Isotype
    IgG
  • Application Notes
    Anti-Penicillinase antibody has been assayed against 1.0 µg of Penicillinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Penicillinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:20.000
    IF Immunoprecipitation Dilution: 1:100
    Western Blot Dilution: 1:500 - 1:5.000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Handling Advice
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Expiry Date
    Expiration date is one (1) year from date of opening.
  • Target
    Penicillinase
    Abstract
    Penicillinase Products
    Synonyms
    beta-lactamase TEM-1 antibody, blaTEM-1 antibody
    Background
    Anti-Penicillinase antibody recognizes the protein penicillinase, a beta lactamase specific to penicillins. Penicillinase hydrolyzes the beta-lactam ring of penicillins destroying their antibacterial potency. Thus, penicillinase is often found in antibiotic-resistant bacteria. Synonyms: Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody
    Gene ID
    9125732
    UniProt
    P05364
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