CES3 antibody (Biotin)

Catalog No. ABIN1607803

Supplier Product No.: 200-4669-0100

Product Details anti-CES3 Antibody (Biotin)

Target: CES3 (Carboxylesterase 3 (CES3))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CES3 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Supplier Product No.: 200-4669-0100

Supplier: Rockland

Purpose: Esterase Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver].

Characteristics: Synonyms: rabbit anti-Esterase Antibody biotin Conjugation, biotin conjugated rabbit anti-Esterase Antibody, Liver carboxylesterase, Proline-beta-naphthylamidase, Retinyl ester hydrolase

Purification: Anti-Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Esterase [Porcine Liver]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Esterase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Esterase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: User Optimized

ELISA Dilution: 1:2,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CES3

Target: CES3 (Carboxylesterase 3 (CES3))

Alternative Name: CES3 (CES3 Products)

Background: Background: Anti-Esterase antibody is involved in the detoxification of xenobiotics, as well as in the activation of ester and amide prodrugs. The antibody is inactive on long-chain triacylglycerols and diacylglycerol, but active towards triacylglycerides composed with short-chain fatty acids from C2 to C6 and monoacylglycerols containing fatty acids from C2 to C12. Esterase hydrolyzes aromate and alkyl esters and vitamin A acetate, the rate of hydrolysis varies due to the hydrolyzing substances. Anti-Esterase antibody member of the type-B carboxylesterase/lipase family.

Gene ID: 100626873, 343791015

UniProt: Q29550

Pathways: Monocarboxylic Acid Catabolic Process