CPA antibody (Biotin)

Catalog No. ABIN1607785

Product Details anti-CPA Antibody (Biotin)

Target: CPA (Carboxypeptidase A (CPA))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPA antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Carboxypeptidase A from other sources may occur but have not been specifically determined.

Purity: Anti-Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas].

Endotoxin Level: Low Endotoxin : No

Immunogen: Carboxypeptidase A [Bovine Pancreas]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Carboxypeptidase A has been assayed against 1.0 µg of Carboxypeptidase A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15.000 to 1:55.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for CPA

Target: CPA (Carboxypeptidase A (CPA))

Alternative Name: Carboxypeptidase A (CPA Products)

Background: Anti-Carboxypeptidase A antibody catalyzes the release of C-terminal amino acids with little or no action with –Asp, -Glu, -Arg, -Lys or –Pro. This antibody is ideal for investigators interested in Metabolism, Cancer, Signal Transduction, or Cell Biology research. Synonyms: Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody

Gene ID: 286762

UniProt: P00730