Amylase, alpha antibody (Biotin)

Catalog No. ABIN1607777

Product Details anti-Amylase, alpha Antibody (Biotin)

Target: Amylase, alpha (AMY)

Reactivity: Bacillus amyloliquefaciens

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Amylase, alpha antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against a-Amylase from other sources may occur but has not been specifically determined.

Purity: Anti-Alpha Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified a-Amylase [B.amyloliquefaciens].

Endotoxin Level: Low Endotoxin : No

Immunogen: a-Amylase [Bacillus amyloliquefaciens]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Alpha Amylase has been assayed against 1.0 µg of a-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Amylase, alpha

Target: Amylase, alpha (AMY)

Alternative Name: Alpha Amylase (AMY Products)

Synonyms: LOC663954 antibody, LOC100136478 antibody, alpha amylase antibody, alpha-amylase antibody, LOC663954 antibody, HQ_RS05305 antibody, LOC100136478 antibody

Background: Alpha Amylase is a member of the glycosyl hydrolase 13 family, involved primarily in the hydrolysis of biopolymer residues of 1, 4-alpha-D-glucosidic linkages in polysaccharides. It performs its catalytic function in metabolism of carbohydrates. Anti-Alpha Amylase antibody is ideal for investigators involved in Metabolism, Signal Transduction, and Cancer research. Synonyms: Alpha-amylase EC=3.2.1.1 1,4-alpha-D-glucan glucanohydrolase

Gene ID: 7849308

UniProt: P00692