This Aldehyde Dehydrogenase antibody is conjugated to Biotin
Application
Western Blotting (WB), ELISA, Immunoprecipitation (IP)
Cross-Reactivity (Details)
Cross reactivity against Aldehyde Dehydrogenase from other sources may occur but have not been specifically determined.
Purity
Anti-Aldehyde Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Aldehyde Dehydrogenase [Yeast].
Endotoxin Level
Low Endotoxin : No
Immunogen
Aldehyde Dehydrogenase [Yeast] Immunogen Type: Native Protein
Anti-Aldehyde Dehydrogenase has been assayed against 1.0 µg of Aldehyde Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1.000 to 1:5.000 of the reconstitution concentration is suggested for this product. ELISA Dilution: 1:5.000 - 1:20.000 IF Immunoprecipitation Dilution: 1:100 Western Blot Dilution: 1:500 - 1:5.000
Restrictions
For Research Use only
Format
Liquid
Concentration
10 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Handling Advice
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Storage
-20 °C
Storage Comment
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Expiry Date
Expiration date is one (1) year from date of opening.
Aldehyde Dehydrogenase catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. It is active on a variety of saturated and unsaturated aliphatic aldehydes from 6 to 24 carbons in length. Anti-Aldehyde Dehydrogenase antibody is ideal for researchers interested in Metabolism, Signal Transduction, Cardiovascular, or Neuroscience research. Synonyms: Aldehyde dehydrogenase [NAD(P)+] 1 ALD2, ALD5