Alcohol Dehydrogenase (ADH) antibody (Biotin)

Catalog No. ABIN1607774

Product Details

Target: Alcohol Dehydrogenase (ADH)

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have not been specifically determined.

Purity: Anti-Alcohol Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast].

Endotoxin Level: Low Endotoxin : No

Immunogen: Alcohol Dehydrogenase [Yeast]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Alcohol Dehydrogenase has been assayed against 1.0 µg of Alcohol Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details

Target: Alcohol Dehydrogenase (ADH)

Alternative Name: Alcohol Dehydrogenase (ADH Products)

Synonyms: ADH antibody, ALCOHOL DEHYDROGENASE antibody, ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE antibody, ATADH antibody, ATADH1 antibody, F22K20.19 antibody, F22K20_19 antibody, alcohol dehydrogenase 1 antibody, 2610201A18Rik antibody, Akr1a4 antibody, ALR1 antibody, Adh antibody, Adh1a antibody, Adh1c antibody, aldo-keto reductase family 1 member A1 antibody, alcohol dehydrogenase 1 antibody, aldo-keto reductase family 1, member A1 (aldehyde reductase) antibody, alcohol dehydrogenase 1 (class I) antibody, AKR1A1 antibody, ADH1 antibody, Akr1a1 antibody, Adh1 antibody

Background: Alcohol Dehydrogenase is part of the zinc-containing alcohol dehydrogenase family. Anti-Alcohol Dehydrogenase catalyzes primary branched alcohols to convert to their aldehyde forms and shows some reactivity with secondary alcohols. Localized in the cytoplasm, the Anti-Alcohol Dehydrogenase antibody is ideal for researchers interested in Metabolism, Signal Transduction, and Cancer research. Synonyms: Alcohol dehydrogenase 1 EC=1.1.1.1 Alcohol dehydrogenase I YADH-1

Gene ID: 2538902, 12643994

UniProt: P00330