Acid Phosphatase antibody (Biotin)
-
- Target See all Acid Phosphatase (ACP) Antibodies
- Acid Phosphatase (ACP)
-
Reactivity
- Potato
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Acid Phosphatase antibody is conjugated to Biotin
-
Application
- Western Blotting (WB), Dot Blot (DB)
- Supplier Product No.
- 200-4640-0100
- Supplier
- Rockland
- Purpose
- Acid Phosphatase Antibody Biotin Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato].
- Characteristics
- Synonyms: rabbit anti-Acid Phosphatase Antibody biotin Conjugation, biotin conjugated rabbit anti-Acid Phosphatase Antibody, Acid phosphatase 1 soluble antibody, PAP3, Acid phosphatase of erythrocyte antibody, Adipocyte acid phosphatase antibody, Cytoplasmic phosphotyrosyl protein phosphatase antibody, HAAP antibody
- Purification
- Acid Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Immunogen
-
Immunogen: Acid Phosphatase [Potato]
Immunogen Type: Native Protein
- Isotype
- IgG
- Top Product
- Discover our top product ACP Primary Antibody
-
-
- Application Notes
-
Application Note: Anti-Acid Phosphatase Biotin Conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Acid Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:32,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: User Optimized
Other: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 10 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
-
- Target
- Acid Phosphatase (ACP)
- Alternative Name
- Acid Phosphatase (ACP Products)
- Background
- Background: Acid Phosphatase is an enzyme which facilitates hydrolysis of alcohol groups on phosphate esters. Anti-Acid Phosphatase antibodies are ideal for investigators interested in Cancer, Enzyme, Cell Biology, and Cell Cycle Research.
- UniProt
- Q6J5M8
-
