Glucose Oxidase antibody (Biotin)

Catalog No. ABIN1607767

Product Details anti-Glucose Oxidase Antibody (Biotin)

Target: Glucose Oxidase (GOD)

Reactivity: Aspergillus niger

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glucose Oxidase antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB)

Cross-Reactivity (Details): Cross reactivity against Glucose Oxidase from other sources may occur but have not been specifically determined.

Purity: Anti-GLUCOSE OXIDASE Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [A.niger].

Endotoxin Level: Low Endotoxin : No

Immunogen: Glucose Oxidase [A.niger]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-GLUCOSE OXIDASE Antibody has been assayed against 1.0 µg of Glucose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:50.000 to 1:250.000 of the reconstitution concentration is suggested for this product. Anti-GLUCOSE OXIDASE Antibody has been assayed against 1.0 µg of Glucose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:50.000 to 1:250.000 of the reconstitution concentration is suggested for this product. Specific condtions should be optimized by user.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Glucose Oxidase

Target: Glucose Oxidase (GOD)

Alternative Name: Glucose Oxidase (GOD Products)

Synonyms: LOC406081 antibody, GB19418 antibody, glucose oxidase antibody, LOC406081 antibody, AFUA_2G14840 antibody, SS1G_10949 antibody, AOR_1_952194 antibody, AOR_1_786174 antibody, AOR_1_358034 antibody, CC1G_15386 antibody, VDBG_09040 antibody, VDBG_06502 antibody, SCHCODRAFT_113971 antibody

Background: Glucose Oxidase antibody detect the glucose oxidase protein. The glucose oxidase enzyme (GOx) (EC 1.1.3.4) is an oxido-reductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. In cells, it aids in breaking the sugar down into its metabolites. Glucose oxidase is widely used for the determination of free glucose in body fluids (diagnostics), in vegetal raw material, and in the food industry. GOx is a dimeric protein, the 3D structure of which has been elucidated. The active site where glucose binds is in a deep pocket. The enzyme, like many proteins that act outside of cells, is covered with carbohydrate chains. At pH 7, glucose exists in solution in cyclic hemiacetal form as 63.6 % β-D-glucopyranose and 36.4 % α-D-glucopyranose, the proportion of linear and furanose form being negligible. Anti-Glucose Oxidase antibody is ideal for investigators involved in glucose energy metabolism research. Synonyms: Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Gene ID: 2683500

UniProt: P13006