Calf Intestinal Alkaline Phosphatase (CIAP) antibody (Biotin)
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- Target See all Calf Intestinal Alkaline Phosphatase (CIAP) products
- Calf Intestinal Alkaline Phosphatase (CIAP)
- Reactivity
- Cow
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- Western Blotting (WB), ELISA
- Supplier Product No.
- 200-4635-0100
- Supplier
- Rockland
- Purpose
- Alkaline Phosphatase Antibody Biotin Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].
- Characteristics
- Synonyms: rabbit anti-Alkaline Phosphatase Antibody biotin Conjugation, biotin conjugated rabbit anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody
- Purification
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Immunogen
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Immunogen: Alkaline Phosphatase [Calf Intestine]
Immunogen Type: Native Protein
- Isotype
- IgG
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anti-Calf Intestinal Alkaline Phosphatase (CIAP) antibody
CIAP Reactivity: Cow WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Application Note: Anti-Alkaline Phosphatase Biotin Conjugated Antibody has been tested by ELISA and Western blot. This product is assayed against 1.0 μg of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:1,000 - 1:10,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 10 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Calf Intestinal Alkaline Phosphatase (CIAP)
- Alternative Name
- Calf Intenstinal Alkaline Phosphatase (CIAP Products)
- Background
- Background: At a high pH , Anti-Alkaline Phosphatase antibodies strip phosphate groups from DNA, RNA, and proteins. ALP is most abundant in placental and intestinal tissue specific such as the liver, placenta, and bone. Located in the cell membrane, Anti-Alkaline Phosphatase antibodies are ideal for researchers involved in Cancer, Enzyme, Tags and Cell Marker, and Cell Cycle research.
- Gene ID
- 280993
- UniProt
- P19111
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