Ovalbumin antibody (Biotin)

Catalog No. ABIN1607759

Supplier Product No.: 200-4633-0100

Product Details anti-Ovalbumin Antibody (Biotin)

Target: Ovalbumin (OVA)

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ovalbumin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Supplier Product No.: 200-4633-0100

Supplier: Rockland

Purpose: Ovalbumin Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].

Characteristics: Synonyms: rabbit anti-Ovalbumin Antibody biotin Conjugation, biotin conjugated rabbit anti-Ovalbumin antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2, Hen Egg White

Purification: Anti-Ovalbumin Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Ovalbumin [Hen Egg White]

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 10-20

Application Details

Application Notes:

Application Note: Anti-Ovalbumin Antibody Biotin Conjugated is tested by ELISA, dot blot, and western blotting. In western blots anti-ovalbumin detects a single band of the expected apparent molecular weight. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Ovalbumin

Target: Ovalbumin (OVA)

Alternative Name: SERPINB14 (OVA Products)

Background: Background: Ovalbumin Antibody Biotin Conjugated is specific for ovalbumin. Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65 % of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily. The function of ovalbumin is unknown, although it is presumed to be a storage protein or carrier protein.

Gene ID: 396058

UniProt: P01012