Ovalbumin antibody (Biotin)

Catalog No. ABIN1607759

Product Details anti-Ovalbumin Antibody (Biotin)

Target: Ovalbumin (OVA)

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ovalbumin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivities against Ovalbumin from other sources may occur but have not been specifically determined.

Purity: Anti-Ovalbumin Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].

Endotoxin Level: Low Endotoxin : No

Immunogen: Ovalbumin [Hen Egg White]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Ovalbumin Antibody Biotin Conjugated is suitable for both ELISA, and for western blotting. In western blots anti-ovalbumin detects a single band of the expected apparent molecular weight. Researchers should determine optimal titers for applications that are not stated below.
ELISA Dilution: 1:30.000
Fluorochrome Protein Value: 10-20
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Ovalbumin

Target: Ovalbumin (OVA)

Alternative Name: Ovalbumin (OVA Products)

Background: Ovalbumin Antibody Biotin Conjugated is specific for ovalbumin. Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65 % of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily. The function of ovalbumin is unknown, although it is presumed to be a storage protein or carrier protein. Synonyms: Ovalbumin, Egg albumin. Plakalbumin, Allergen Gal d II, Allergen=Gal d 2

Gene ID: 396058

UniProt: P01012