Dextranase antibody (Biotin)
Reactivity: Penicillium sp.
WB, ELISA, IP
Host: Rabbit
Polyclonal
Biotin
-
- Target See all Dextranase products
- Dextranase
- Reactivity
- Penicillium sp.
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Dextranase antibody is conjugated to Biotin
-
Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Cross-Reactivity (Details)
- Cross reactivity against Dextranase from other sources is unknown.
- Purity
- Anti-Dextranase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species].
- Endotoxin Level
- Low Endotoxin : No
- Immunogen
-
Dextranase [Penicillium species] DEX
Immunogen Type: Native Protein - Isotype
- IgG
-
anti-Dextranase antibody
Reactivity: Penicillium sp. ELISA Host: Rabbit Polyclonal unconjugated
-
- Application Notes
-
Anti-Dextranase has been assayed against 1.0 µg of Dextranase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3.000 to 1:14.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Dextranase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3.000 to 1:14.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000 - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 10 mg/mL
- Buffer
-
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - Handling Advice
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- Expiry Date
- Expiration date is one (1) year from date of opening.
-
- Target
- Dextranase
- Abstract
- Dextranase Products
- Synonyms
- dextranase antibody, Dextranase antibody, Teth39_0264 antibody, Teth514_0757 antibody, VDBG_04792 antibody, Thet_2161 antibody, Thebr_0271 antibody
- Background
- Anti-Dextranase antibody is a transferase enzyme which catalyzes the synthesis of dextran by engaging in the endohydrolysis of 1, 6 alpha-D-glucosidic linkages in dextran. Belonging to the glycosyl hydrolase 49 family, Anti-Destranase antibody is ideal for researchers interested in Metabolism and Signal Transduction research. Synonyms: Dextranase Alpha-1,6-glucan-6-glucanohydrolase DEX
- UniProt
- P48845
-
You are here:
