beta-2 Microglobulin antibody

Catalog No. ABIN1607703

Supplier Product No.: 200-401-141-0100

Product Details anti-beta-2 Microglobulin Antibody

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 200-401-141-0100

Supplier: Rockland

Purpose: Beta-2-Microglobulin Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum, as well as purified and partially purified b2-Microglobulin (Human Urine).

Characteristics: Synonyms: rabbit anti-Beta-2-Microglobulin Antibody, b2-Microglobulin, B2M antibody, B2MG_HUMAN antibody, Beta 2 microglobin antibody, Beta 2 microglobulin precursor antibody, Beta chain of mhc class 1 proteins antibody, Hdcma22p antibody, CDABP0092 antibody, IMD43 antibody

Purification: Anti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with human beta-2-Microglobulin protein isolated from urine.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Anti-beta-2-Microglobulin antibody has been tested in western blot, immunohistochemistry, and immunofluorescence. This product detects a single band of the expected apparent molecular weight (~13.7 kDa), and is suitable for use in ELISA. Researchers should determine optimal titers for applications that are not stated below.

Western Blot Dilution: 1:200 - 1:500

ELISA Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: 1:200

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: B2M (B2M Products)

Background: Background: Anti-beta-2-Microglobulin Antibody detects beta-2-Microglobulin. Beta-2-microglobulin is a component of the class I major histocompatibility complex (MHC), which are present on all nucleated cells (excludes red blood cells). It is involved in the presentation of peptide antigens to the immune system. Beta-2-microglobulin associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules such as CD1 and Qa. Defects in B2M are the cause of hypercatabolic hypoproteinemia. Anti-beta-2-Microglobulin Antibody is ideal for investigators involved in Cell Signaling, Immunology and Cell Biology research.

Gene ID: 567

NCBI Accession: NP_004039

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process