IdU antibody

Catalog No. ABIN1607683

Product Details anti-IdU Antibody

Target: IdU (Iododeoxyuridine (IdU))

Reactivity: Chemical

Host: Mouse

Clonality: Monoclonal

Conjugate: This IdU antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Flow Cytometry (FACS)

Cross-Reactivity (Details): Cross reactivity is not observed with BrdU, CldU or FdU.

Purity: Anti-IdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography. This antibody is specific for IdU.

Endotoxin Level: Low Endotoxin : No

Immunogen: Anti-IdU mononclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
Immunogen Type: Other

Clone: 32D8-D9

Application Details

Application Notes: Anti-IdU Antibody has been tested as suitable for western blot, ELISA, and immunofluorescence microscopy. This antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incoporated IdU thymidine analog from replicated cells.
ELISA Dilution: 1:1000-1:10000
Western Blot Dilution: 1:2000 - 1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage: 4 °C/-20 °C

Storage Comment: Store IdU Antibody at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: Expiration date is one (1) year from date of opening.

References for anti-IdU antibody (ABIN1607683)

Alén, Gómez-Redondo, Rivera, Suárez, Ramos-Ibeas, Pericuesta, Fernández-González, Perez-Cerezales, Horiuchi, Orio, Rodriguez de Fonseca, Gutiérrez-Adán: "Sex-Dimorphic Behavioral Alterations and Altered Neurogenesis in U12 Intron Splicing-Defective Zrsr1 Mutant Mice." in: International journal of molecular sciences, Vol. 20, Issue 14, (2019) (PubMed).

Target Details for IdU

Target: IdU (Iododeoxyuridine (IdU))

Abstract: IdU Products

Target Type: Chemical

Background: Iododeoxyuridine (5-Iodo-2'-deoxyuridine, IdU) is a synthetic thymidine nucleoside analog. IdU, like BrdU thymidine analog, is used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and IdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for IdU are subsequently used to detect the incorporated IdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat. Synonyms: Iododeoxyuridine, 5-iodo-2'-deoxyuridine, IdU