BrdU antibody

Catalog No. ABIN1607682

Supplier Product No.: 200-301-h50

Product Details anti-BrdU Antibody

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Supplier Product No.: 200-301-h50

Supplier: Rockland

Purpose: BrdU Antibody

Cross-Reactivity (Details): This antibody reacts strongly with BrdU.

Characteristics: Synonyms: mouse anti-BrdU Antibody, Bromodeoxyuridine, 5-bromo-2'-deoxyuridine, BrdU

Purification: Anti-BrdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.

Immunogen Type: Other

Clone: 29G6-E8

Isotype: IgG1 lambda

Application Details

Application Notes:

Flow Cytometry Dilution: 1:50-1:100

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-BrdU Antibody has been tested as suitable for immunofluorescence and immunoblot assays. Antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Antibody will detect incorporated BrdU thymidine analog from replicated cells.

Western Blot Dilution: 1:2000 - 1:5000

ELISA Dilution: 1:2000 - 1:10000

IF Microscopy Dilution: 1:500-1:3000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store BrdU Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-BrdU antibody (ABIN1607682)

Silva, Peyre, Adhikari, Tielens, Tanco, Van Damme, Magno, Krusy, Agirman, Magiera, Kessaris, Malgrange, Andrieux, Janke, Nguyen: "Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis." in: Cell, Vol. 172, Issue 5, pp. 1063-1078.e19, (2019) (PubMed).

Wang, Shen, Cheng, Deng, Engelhardt, Yan, Qiu: "Parvovirus Expresses a Small Noncoding RNA That Plays an Essential Role in Virus Replication." in: Journal of virology, Vol. 91, Issue 8, (2017) (PubMed).

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU (BrdU Products)

Target Type: Chemical

Background: Background: Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for BrdU are subsequently used to detect the incorporated BrdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.