Lactate Dehydrogenase antibody (Biotin)

Catalog No. ABIN1607671

Product Details anti-Lactate Dehydrogenase Antibody (Biotin)

Target: Lactate Dehydrogenase (LDH)

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This Lactate Dehydrogenase antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Lactate Dehydrogenase from other sources may occur but have not been specifically determined.

Purity: Anti-lactate dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].

Endotoxin Level: Low Endotoxin : No

Immunogen: Lactate Dehydrogenase [full length protein isolated from rabbit muscle]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-lactate dehydrogenase antibody has been assayed against 1.0 µg of Lactate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

References for anti-Lactate Dehydrogenase antibody (Biotin) (ABIN1607671)

Vincent, Cornea, Jong, Laferrière, Kumar, Mickeviciute, Fung, Bandegi, Ribeiro-da-Silva, OMalley, Coderre: "Intracellular mGluR5 plays a critical role in neuropathic pain." in: Nature communications, Vol. 7, pp. 10604, (2016) (PubMed).

Target Details for Lactate Dehydrogenase

Target: Lactate Dehydrogenase (LDH)

Alternative Name: Lactate Dehydrogenase (LDH Products)

Synonyms: CG10160 antibody, Dmel\\CG10160 antibody, IMP-L3 antibody, IMPL3 antibody, Imp-L3 antibody, Impl3 antibody, LDH antibody, Ld antibody, Ldh antibody, dLdha antibody, dmLDH antibody, Ecdysone-inducible gene L3 antibody, lactate dehydrogenase antibody, ldh2 antibody, ImpL3 antibody, ldh antibody, ldh2 antibody, Ldh antibody, LDH-A antibody

Background: Anti-lactate dehydrogenase recognizes lactate dehydrogenase which catalyzes the interconversion of pyruvate to lactate and NADH to NAD+. This reaction occurs in the absence or lack of oxygen, making lactate dehydrogenase critical for anerobic respiration. There five known isozymes of LDH, each localized in different types of tissue. In medicine, LDH is often used as a marker of various diseases ranging from hemolysis to encephalitis. Anti-lactate dehydrogenase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction. Synonyms: L-lactate dehydrogenase A chain LDH-A LDH muscle subunit LDH-M

Gene ID: 100009107, 126050

UniProt: P13491