Pyranose Oxidase antibody (Biotin)

Catalog No. ABIN1607646

Product Details anti-Pyranose Oxidase Antibody (Biotin)

Target: Pyranose Oxidase

Reactivity: Microbial

Host: Goat

Clonality: Polyclonal

Conjugate: This Pyranose Oxidase antibody is conjugated to Biotin

Application: Immunoprecipitation (IP), Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Pyranose Oxidase from other sources is unknown.

Purity: Anti-Pyranose oxidase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyranose Oxidase [E.coli].

Endotoxin Level: Low Endotoxin : No

Immunogen: Pyranose Oxidase [E.coli]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Pyranose oxidase antibody has been assayed against 1.0 µg of Pyranose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:70.000 to 1:350.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Pyranose Oxidase

Target: Pyranose Oxidase

Abstract: Pyranose Oxidase Products

Synonyms: Pyranose oxidase antibody, pyranose oxidase antibody, Achl_3529 antibody, Kvar_1112 antibody, AMED_5392 antibody

Background: Anti-Pyranose oxidase recognizes the oxidoreductase pyranose oxidase. In general, pyranose catalyzes the oxidation of aldopyranoses at the carbon 2 position to form 2-ketoaldoses . Notably, pyranose oxidase catalyzes the conversion of D-glucose and oxygen to 2-dehydro-D-glucose and hydrogen peroxide using flavin adenine dinucleotide (FAD) as a cofactor. Pyranose oxidase also plays a role in the pentose phosphate pathway. Synonyms: P2Ox Pyranose oxidase PROD POD POx EC=1.1.3.10 Pyranose:oxygen 2-oxidoreductase Glucose 2-oxidase FAD-oxidoreductase

UniProt: Q5G234, P79076