Glucoamylase antibody (Biotin)

Catalog No. ABIN1607644

Product Details anti-Glucoamylase Antibody (Biotin)

Target: Glucoamylase

Reactivity: Rhizopus

Host: Goat

Clonality: Polyclonal

Conjugate: This Glucoamylase antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity against Glucoamylase from other sources is unknown.

Purity: Anti-Glucoamylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glucoamylase [Rhizopus].

Endotoxin Level: Low Endotoxin : No

Immunogen: Glucoamylase [Rhizopus]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Glucoamylase has been assayed against 1.0 µg of Glucoamylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15.000 to 1:75.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Glucoamylase

Target: Glucoamylase

Abstract: Glucoamylase Products

Synonyms: An03g06550 antibody, AO090003000321 antibody, AO090010000746 antibody, glucoamylase antibody, SACE_7136 antibody, UREG_05278 antibody, RR_RS12725 antibody, ANI_1_820034 antibody, AOR_1_552154 antibody, AOR_1_1262024 antibody, PAAG_02688 antibody, MCYG_06367 antibody, VDBG_01600 antibody, VDBG_06382 antibody, MGYG_06815 antibody, TERG_07238 antibody

Background: Anti-Glucoamylase antibody recognizes the enzyme glucoamylase. The catalytic domain of glucoamylase hydrolyzes terminal 1,4-alpha-D-glucose from non-reducing ends and releases beta-glucose. Synonyms: 1 4 alpha D glucan glucohydrolase antibody, Amyloglucosidase antibody, Gluc 1 antibody, Gluc 2 antibody, Gluc 3 antibody, Glucan 1 4 alpha glucosidase antibody, Glucan 14 alpha glucosidase antibody

UniProt: P07683