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Glucoamylase antibody (Biotin)

Reactivity: Rhizopus WB, ELISA, IP Host: Goat Polyclonal Biotin
Catalog No. ABIN1607644
  • Target See all Glucoamylase products
    Glucoamylase
    Reactivity
    • 15
    • 2
    Rhizopus
    Host
    • 15
    • 2
    Goat
    Clonality
    • 17
    Polyclonal
    Conjugate
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Glucoamylase antibody is conjugated to Biotin
    Application
    • 16
    • 13
    • 13
    • 5
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Cross-Reactivity (Details)
    Cross reactivity against Glucoamylase from other sources is unknown.
    Purity
    Anti-Glucoamylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glucoamylase [Rhizopus].
    Endotoxin Level
    Low Endotoxin : No
    Immunogen
    Glucoamylase [Rhizopus]
    Immunogen Type: Native Protein
    Isotype
    IgG
  • Application Notes
    Anti-Glucoamylase has been assayed against 1.0 µg of Glucoamylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15.000 to 1:75.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:20.000
    IF Immunoprecipitation Dilution: 1:100
    Western Blot Dilution: 1:500 - 1:5.000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Handling Advice
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Expiry Date
    Expiration date is one (1) year from date of opening.
  • Target
    Glucoamylase
    Abstract
    Glucoamylase Products
    Synonyms
    An03g06550 antibody, AO090003000321 antibody, AO090010000746 antibody, glucoamylase antibody, SACE_7136 antibody, UREG_05278 antibody, RR_RS12725 antibody, ANI_1_820034 antibody, AOR_1_552154 antibody, AOR_1_1262024 antibody, PAAG_02688 antibody, MCYG_06367 antibody, VDBG_01600 antibody, VDBG_06382 antibody, MGYG_06815 antibody, TERG_07238 antibody
    Background
    Anti-Glucoamylase antibody recognizes the enzyme glucoamylase. The catalytic domain of glucoamylase hydrolyzes terminal 1,4-alpha-D-glucose from non-reducing ends and releases beta-glucose. Synonyms: 1 4 alpha D glucan glucohydrolase antibody, Amyloglucosidase antibody, Gluc 1 antibody, Gluc 2 antibody, Gluc 3 antibody, Glucan 1 4 alpha glucosidase antibody, Glucan 14 alpha glucosidase antibody
    UniProt
    P07683
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